甘肃裕固族HLA-DRB1基因多态性及其族源分析

The Polymorphism on HLA-DRB 1 Locus of Gansu Yugu Ethnic Group and the Analysis of Its Origin

目的:研究甘肃裕固族HLA-DRB1基因的多态性,探讨裕固族的起源、迁徙及其与其他民族的关系。方法:应用PCR-SSP基因分型技术,对54例裕固族个体进行了HLA-DRB1位点的基因分型并进行相应等位基因频率的比较。结果:甘肃裕固族HLA-DRB1位点共检出了14种等位基因,其中高频基因为DR5(0.2115),DR4(0.1346)和DR7(0.1250),低频的等位基因为DR14(0.0096)和DR13(0.0192);裕固族人HLA-DRB1座位等位基因总的分布格局与蒙古族最接近,与云南黎族则相差较远。结论:对裕固族和我国各地人群的HLA-DRB1频率进行了聚类分析,极为相似的HLA-DRB1背景提示裕固族和蒙古族之间密切的遗传关系。

Objective： To genetically explore the origin and migration of Gansu Yugu and its relationship among main surrounding ethnic groups. Method： The HLA-DRB 1 locus was genotyped by polymerase chain reaction-sequence specific primer （PCR-SSP） technique for 54 healthy, unrelated, randomly selected Yugu adults whose families had lived in Gansu for more than 3 generations and every allelic frequency was comparatively analyzed with that of some main Chinese populations. Results： The most common alleles among the detected 14 alleles were DR5 （0.2115）, DR4 （0.1346） and DR7 （0.1250）. The frequencies of DR14 （0.0096） 和 DR13 （0.0192） are very low although detected. The overall alleles distribution profile on HLA-DRB1 of Yugu were much dramatically closed to those of Mongolian, and far away from that of Li of Yunnan. Conclusion： It is concluded that there is a very close genetic relationship between Yugu and Mogolian people according to their extremely similar background on HLA-DRB 1 polymorphism.