溃疡性结肠炎和肠易激综合征患者结肠黏膜HBD-2和TNF-α的表达

Expression of human β-defensin 2 and tumor necrosis factor -α in colonic mucosae of ulcerative colitis and diarrhea-predominant irritable bowel syndrome

目的研究β-防御素2（HBD-2）和肿瘤坏死因子α（TNF-α）在溃疡性结肠炎和肠易激综合征（腹泻型）患者结肠黏膜中的表达，及其在发病中的作用和相互关系。方法收集30例溃疡性结肠炎患者、20例肠易激综合征（腹泻型）患者和10名健康对照的结肠黏膜组织，免疫组织化学法检测各组HBD-2和TNF—α蛋白表达。结果免疫组织化学分析显示溃疡性结肠炎组HBD-2和TNF—α表达强度均高于肠易激综合征（腹泻型）组（z=-4．856，z=-3．987，均P〈0．01）和正常结肠黏膜组（z=-3．611，z=-3．248，均P〈0．01），肠易激综合征（腹泻型）组和正常结肠黏膜组HBD-2和TNF—α表达强度比较差异无统计学意义（z=-0．373，z=-0．032，均P〉0．05）。轻、中、重度3组溃疡性结肠炎之间HBD-2和TNF—α的表达强度比较差异无统计学意义（Χ^2=1．190、P〉0．05，Χ^2=1．672、P〉0．05）。结论HBD-2和TNF—α的表达水平在溃疡性结肠炎结肠黏膜组织中明显升高，与肠易激综合征（腹泻型）关系不密切，与溃疡性结肠炎病情轻重关系不密切。

Objective To detect the expression of human β-defensin 2 （HBD-2） and tumor necrosis factor-α （TNF-α） in the colonic mucosae of ulcerative colitis （UC） patients and diarrhea- predominant irritable bowel syndrome （IBS-D） patients and to evaluate the roles of HBD-2 and TNF-α in the pathogenesis and the relationship between them. Methods Immunohistochemistry was conducted on the biopsy samples of colonic mucosa from 30 UC patients, 20 IBS-D patients, and 10 normal persons to measure the expression of HBD-2 and TNF-α. Results The expression levels of HBD-2 and TNF-α in the colonic mucosa of UC were both significantly higher than those in the colonic mucosa of IBS-D （z = - 4. 856 ,z = - 3. 987, all P 〈 0.01 ） and in the normal colonic mucosa （z = - 3.611 ,z = - 3. 248, all P 〈 0.01 ）. But no significant differences were found between the colonic mucosa of IBS-D and the normal colonic mucosa in the intensity of the expressions of HBD-2 and TNF-α （z = -0. 373 ,z = -0.032, all P 〉 0.05 ）. There were no significant differences in the intensity of the expressions of HBD-2 and TNF-α in colonic mucosa of among the mild, moderate, and severe UC （Χ^2 = 1. 190,P 〉 0.05, Χ^2 = 1. 672, P 〉 0. 05）. Conclusion The expressions of HBD-2 and TNF-α are significantly increased in UC, but are not correlated with IBS-D and the severity of clinical symptoms of UC patients.