北方棉区棉花黄萎病菌RAPD分析

RAPD Analysis of Verticillium dahliae from Cotton in Northern China.

以１４个黄萎病菌代表菌系为对照，对来自我国北方棉区的３４个棉花黄萎病菌菌系进行ＲＡＰＤ分析。选用对所有供试菌系都有扩增条带的１４个引物，取其结果中稳定性和多态性均好的６５条谱带作类平均法系统聚类分析，建立树状图。将上述４８个菌系分为４大类，结果表明北方棉区河北、河南、山东的部分棉田存在黄萎病菌落叶型菌系的危害，而且８５．７％的落叶型菌系与对照的美国落叶型菌系Ｔ９、Ｖ４４的亲缘关系比与对照的江苏落叶型菌系ＶＢ、Ｖ９９１更接近。

With 14 representative strains of Verticillium dahliae as check, 34 strains of V. dahliae from cotton in Northern China were analysised by method of random amplified polymorphic DNA (RAPD). Average linkage cluster analysis based on 65 ploymorphic bands from results amplified with 14 effective primers was used to generate a dendrogram. 48 strains tested were clustered into 4 groups. It was indicated that defoliating strains of V. dahliae had been in Northern China and the relationship between 85 7% defoliating strains tested and control defoliating strains from America were closer than from Jiangsu Province.