HPLC法测定卡络磺钠葡萄糖注射液的含量

Determination of the Content of Carbazochrome Sodium Sulfonate and Glucose Injection by HPLC

采用Kromasil-C18色谱柱(250×4.6mm,5μm),流动相为0.12%磷酸二氢铵溶液(用磷酸调节pH值至3)-无水乙醇(85∶15),检测波长为363nm,流速为1.0mL/min,HPLC法测定卡络磺钠葡萄糖注射液含量。结果表明,卡络磺钠在0.080—0.120mg/mL浓度范围内,峰面积与浓度呈良好的线性关系(r=0.9995),平均回收率为100.35%。所建方法简便,专属性及精密度好,可用于卡络磺钠葡萄糖注射液的含量测定。

Carbazochrome sodium sulfonate and glucose injection was determined by HPLC with Kromasil-C8 column （250 × 4. 6mm,5μm） 0.12%NH4H2PO4 （pH3.0）-ethanol （85 ： 15）as mobile phase detected at 363nm. The linear range of carbazochrome sodium sulfonate was 0. 080--0. 120 （mg/mL） （r=0. 9995）. The average recovery was 100.35%. This method is simple , selective and reproducible for the assay of carbazochrome sodium sulfonate