2个水仙栽培品种离体培养诱导技术研究

Technology of Vitro Induction of Bulb from 2 Varieties of Narcissus

以水仙两个栽培品种Fortissimo、Pink Charm为试验材料,以带鳞茎基盘的双鳞片为外植体,研究了水仙的组织培养诱导技术。结果表明:适合的基本培养基为MS,蔗糖浓度为30g/L,光照条件为12h,外加500ml/L的水解乳蛋白。Fortissimo最适的激素水平为BA3.0mg/L+NAA0.5mg/L+IAA1.0mg/L;Pink Charm为BA3.0mg/L+NAA0.5mg/L+IAA0.5mg/L。

The duble scales with base rootstock from 2 varieties of Narcissus were cultured in vitro to develop induction technology of rapid propagation . The experimental results showed that the proper medium for both Fortissimo and Pink Charm was MS ＋ 30 g/L sucrose ＋ 500 mg/L LH with 12 h light. And the proper hormone to Fortissimo was BA 3.0 mg/L ＋ NAA 0.5 mg/L ＋ IAA 1.0 mg/L, while to Pink Charm was BA 3.0 mg/L ＋ NAA 0.5 mg/L ＋ IAA 0.5 mg/L.