丰隆穴对高脂血症大鼠不同脏器SOD、MDA的影响

Effects of electroacupuncture at Fenglong(ST 40)on SOD and MDA in different organs of the hy-perlipemia rat

目的:探讨丰隆穴的作用靶器官及其化痰作用的机制。方法:采用高脂饮食饲料喂养制备高脂血症大鼠模型。将30只SD大鼠随机分为空白组、模型组、丰隆组,每组10只。空白组每天给予基础饲料喂养,其余2组每天给予高脂饲料喂养。喂养2 w后丰隆组给予电针"丰隆"穴,每周2次,共治疗10次。治疗结束后,检测各脏器超氧化物歧化酶(SOD)活性与丙二醛(MDA)含量。结果:针刺"丰隆"穴使脾脏、肺脏SOD的活性显著升高,肺脏的MDA含量明显降低;有降低肝脏SOD活性和增高MDA含量的趋势;对胰腺的SOD活性、MDA含量影响不显著。结论:在高脂血症情况下,"丰隆"穴调节SOD与MDA的靶点主要在脾、肺、肝脏,尤其对脾、肺脏的影响显著,对胰腺的影响不大。说明丰隆的化痰作用与促进特定脏器的自由基代谢相关。

Objective To probe into the target organ of Fenglong （ST 40） and the mechanism in resolving phlegm. Methods Thirty SD rats were randomly divided into 3 groups, a blank control group, a model group and a Fenglong group, 10 rats in each group. The hyperlipemia rat model was prepared by feeding high fat forage. The blank control group were fed by basic forage each day, and other 2 groups were fed with high fat forage each day. After the rats were fed for 2 weeks, EA was given at ＂Fenglong＂ （ST 40） in the rats of the Fenglong group, twice each week, for 10 times. At the end of EA treatment, SOD activities and MDA contents in the spleen, lung, liver and pancreas were detected. Results Acupuncture at ＂Fenglong＂ （ST 40） significantly increased SOD activities in the spleen and lung, decreased MDA content in the lung, and had a tendency to decrease SOD activity and to increase MDA content in the liver, while it did not significantly influence SOD activity and MDA content in the pancreas. Conclusion The target organs of ＂Fenglong＂ （ST 40） regulating SOD activity and MDA content are spleen, lung and liver, particularly, spleen and lung, with no relation with pancreas. It is indicated that the resolving phlegm function of ＂Fenglong＂ （ST 40） is related with promoting metabolism of free radiations in special organs.