动态超声萃取分光光度法在线测定红花中的总红花黄色素

Determination of Total Safflower Yellow Pigments in Carthamus tinctorius L.by Dynamic Ultrasonic Extraction Coupled with On-line Spectrophotometric Detection

用动态超声萃取分光光度法在线测定了红花中的红花黄色素,对萃取条件进行了优化.萃取与测定同时进行,大大缩短了样品的分析时间,简化了分析过程.通过与静态超声萃取、振荡萃取和索式萃取进行比较,不仅证明该方法耗时最短,而且样品用量小,萃取产率较高.实际样品分析得到了满意的结果.

A dynamic ultrasonic extraction （DUE） coupled with on-line detection by spectrophotometry was proposed for the determination of total safflower yellow pigments （ calculated against safflor yellow A ） in Carthamus tinctorius L. The extraction was performed in a common self-made extraction vessel placed inside an ultrasonic bath and a peristaltic pump was used to deliver the solvent. Several parameters of DUE, including flow rate of extraction solvent, ultrasonic power and sample amount, were optimized. Compared to the off-line method, the on-line approach has the advantages of minimum sample amount（5 mg）, on-line monitoring the extraction process and time-saving（ 10 min） and the method is more convenient for rapid optimization of the extraction process.