金地鼠致癌模型PCNA、BrdU核标记观察结果相关分析

Correlative analysis with proliferating cell nuclear antigen and bromodeoxyuridine on golden hamster carcinogenesis model

癌变的早期诊断对提高疗效具有特殊意义。为寻求简捷、高效、超前、可靠的诊断口腔粘膜癌前病变的方法，实验以DMBA（二甲基苯并蒽）诱导的金地鼠颊囊致癌模型为对象，采用PCNA（增殖细胞核抗原）、BrdU（溴脱氧尿嘧啶）核标记技术手段检测颊囊粘膜组织，并与经典的光镜观察诊断作对照。对三种观察结果行相关分析，发现三者间有高度显著的相关性，证实免疫组化方法在癌变诊断中的实用价值。实验还发现PCNA和BraU检测结果比较敏感、简捷，可靠性较好，因而是有希望的早期诊断癌变的手段之一。

The early diagnosis of carcinomatous conversion has special significance on incerasing therapeutic effectiveness. Proliferating cell nuclearnatigen (PCNA), bromodexoyuridine (BrdU) (immunohistochemistry ways ), and microscopic diagnosis were applied andobserved on golden hamster cheek is Pouch carcinogenisis model induced by dimethylbenzanthracene (DMBA) in order to seek simple,effective,leading and reliable diagnostic way. The results showed that three observable means had significant correlativity, and demonstratedthat immunohistochemistry ways have practical value in diagnosis of carcinomatous conversion. This study suggests that PCNA and BrdU be more sensitive, simple and reliable, andhopeful methods of early diagnosis of carcinomatous conversion.