免疫化疗对口腔鳞癌浸润T淋巴细胞及其亚群的影响

Effect on intratumoral T lymphocyte and its subset in oral squamous cell carcinoma following immunochemotherapy

应用ABC免疫组化技术，检测口腔鳞癌患者接受免疫化疗（S－PVP）和单纯化疗（PVP）治疗前后肿瘤组织内CD3+、CD4+、CD3+和HLA－DR+细胞相对值，比较不同的诱导治疗方案对局部细胞免疫反应的影响．结果显示：S－PVP组治疗前后CD3+、CD4+、CD3+和HLA－DR+细胞分别为65．65、3660、35．93、54．04．和129．26、74．77、38．86、79．64。治疗后CD3+、CD4+细胞较治疗前明显增加（P＜0.05）．PVP组治疗前后CD3+、CD4+、CD3+和HLA－DR+细胞相对值无显著性差异．结果表明，S－PVP免疫化疗可使原发病杜内的T淋巴细胞数量增加，而单纯PVP化疗时局部细胞免疫反应的影响不甚明显。

The relative numbers of CD3+,CDt,CDt and HLA-RD+ cells in the tumor tis-sue before and after inductive therapy was counted using immunohistochemical technique of Avidin Biotin-Peroxidase Complex(ABC),and then the effect on local immune cell following twodifferent inductive therapies was comparatively analysed. The results showed that S-PVP ther-apy could result in significant increasing in numbers of CD and CD cells intratumorally ascompared with pre-therapy (P＜0.05),and in slight increasing in numbers of CD and HLA-DR+ celIs,which is no significant difference between pre-and Post-therapy. In the PVP group,there was no sigificant difference in numbers of CD,CD,CD and HLA-DR cells betweenpre-and post-treatment. The results suggested that T lymphocyte in the tumor site could sig-nificantly increase in numbers following the S-PVP therapy,and PVP chemotherapy did notobviously affect the local cellular immunity.