摘要
目的探讨何首乌饮抗大鼠卵巢组织衰老的机理。方法36只SD大鼠随机分为3组:正常组、模型组、预防组。采用D-半乳糖连续腹腔注射法制造雌性大鼠亚急性衰老模型,预防组在造模的同时以中药方剂何首乌饮灌胃。应用逆转录聚合酶连反应(RT-PCR)方法检测各组大鼠卵巢组织p53、p19ARF、Rb及p16基因的表达,用Western blotting方法检测各组大鼠卵巢组织Rb及p16蛋白的表达情况。结果何首乌饮明显抑制了衰老大鼠卵巢组织中p53、p19ARF、Rb以及p16基因表达的上调趋势(P<0.05),且明显降低了衰老大鼠卵巢组织中Rb及p16蛋白的表达(P<0.05)。结论何首乌饮抑制衰老途径中关键的调节因子p53、p19ARF、Rb及p16的表达,从而起到抗大鼠性腺衰老的作用。
Objective To find the mechanism of traditional Chinese medicine Heshouwuyin（HSWY） in anti-aging in ovary of the aging model rats,so as to provide theoretical and experimental evidence to anti-aging in female sexual gland.Methods Thirty-six rats were divided into three groups at random： normal group,aging model group,prevent aging group.Rats the in prevent aging group were subdivided into four groups： HSWY low dosage group,HSWY medium dosage group,HSWY high dosage group and Heshouwan（HSWW） group.The sub-acutely aging model rats were induced by D-galactose,and rats in the prevent aging group were received intragastric administration of HSWY and HSWW with D-galactose intraperioneal injection.The gene expressions of p53,p19^（ARF）,Rb and p16 were detected in the ovaries of each group with reverse transcription polymerase chain reaction,and the protein expressions of Rb and p16 in the ovaries with Western blotting.Results The model group rats were in the aging status with low spirits and withered and yellowed hair.The ovaries of the rats in the model group expressed aging change.The gene expressions of p53,p19^（ARF）,Rb and p16 were up-regulated and the protein expressions of Rb and p16 were increased in the ovaries of aging rats.HSWY significantly suppressed the gene expressions of p53,p19^（ARF）,Rb and p16（P〈0.05） and depressed the protein expressions of Rb and p16（P〈0.05）.Conclusion HSWY can suppress the expression of key regulators-p53,p19^（ARF）,Rb and p16 in senescent pathway to play a role of anti-aging in rats＇ sexual gland.HSWY fuactions in anti-aging by suppressing the apoptosis gene expressions in the sexual gland of aging rats.
出处
《解剖学报》
CAS
CSCD
北大核心
2008年第2期187-192,共6页
Acta Anatomica Sinica
基金
国家自然科学基金资助项目30572432
