缝隙连接蛋白43在培养的新生大鼠心室肌细胞中的时空表达

SPATIO-TEMPORAL EXPRESSION OF CONNEXIN 43 IN THE CULTURED VENTRICULAR MYOCARDIAL CELLS OF NEONATAL RATS

目的探讨连接蛋白43(Cx43)在培养的新生大鼠心室肌细胞中的时空表达变化规律。方法利用免疫细胞化学方法及免疫电镜技术,观测培养2、4、8、10、12、16、20、26、30d大鼠心室肌细胞Cx43蛋白的表达;结果培养第2d心肌细胞开始表达Cx43,表达部位主要在细胞质中,呈点状;第4d细胞质中点状分布减少,主要集中在细胞膜连接处;第10d在细胞连接处增多,呈条、链状分布;以后基本恒定,无明显变化;第30d时Cx43颗粒出现紊乱分布;结论Cx43在培养的新生大鼠心肌细胞中的表达随培养时间延长而出现位置和量的变化,与培养心肌细胞的生长、发育及功能变化一致。

Objective To investigate the spatio-temporal expression of connexin 43（Cx43） in the cultured ventricular myocardial cells of neonatal rats. Methods The techniques of Immunocytochemistry（ICC） and immuno-electron microscopy were used to detect the Cx43 expression in the cultured rat ventricular myocardial cells on the（2nd）,（4th）,（8th）,（10th）,（12th）,（16th）,（20th,）（26th） and（30th） days. Results Cx43 expression was detected in the cultured ventricular myocardial cells on the 2（nd） day,and the Cx43 granules were located largely in the cellular cytoplasm and membrane.The punctiform granule of the cellular cytoplasm decreased and the expression of Cx43 was located mainly in cellular membrane junction on the 4（th） day.The expression of Cx43 increased in cellular membrane junction on the 10（th） day,and the morphology of Cx43 expression was chain-and strip-like.There were not obvious changes in the following days.The expression of Cx43 on the 30（th） day was derangement.Conclusion The spatio-temporal expression of Cx43 in the cultured ventricular myocardial cells of neonatal rats changed with the cultural time in terms of location and quantity.It was in accordance with the growth and development of the cultured ventricular myocardial cells.