Skp2和P27^(kip1)在膀胱移行上皮细胞癌中的表达及意义

The expression of Skp2 and P27^(kip1) in bladder transitional cell carcinoma and its significance

目的:探讨Skp2和P27kip1在膀胱移行上皮细胞癌(BTCC)中的表达及临床意义。方法:采用免疫组化SP法观察74例BTCC和13例正常膀胱黏膜石蜡标本中Skp2和P27kip1的表达情况,并结合临床资料分析它们的免疫活性在正常膀胱黏膜和膀胱移行上皮细胞癌上表达的差异,以及和肿瘤分级、分期、等病理参数之间的关系。结果:膀胱移行上皮细胞癌中Skp2和P27kip1阳性表达率分别为43.24%和45.95%。Skp2在BTCC中的阳性表达率明显高于正常膀胱黏膜(7.69%),在BTCC中随病理分级升高而升高,但与临床分期无明显的统计学意义,复发组高于初发组,在初法或复发组及瘤体大小组没有明显的统计学意义;P27kip1在BTCC中的阳性表达率低于正常膀胱黏膜(76.92%),并随病理分级、临床分期升高而下降,在初发组高于多复发组,其他两组病理参数组则没有明显的统计学意义;Skp2与P27kip1二者在BTCC中的表达呈负相关性(r=-0.335,P=0.004)并具有统计学意义。结论:Skp2和P27kip1在膀胱移行上皮细胞癌中的表达是判断其生物学行为的重要指标之一,并可能为以后治疗肿瘤提供一个新的靶点。

Objective： To analyze the expression of Skp2 （S-phase kinase-associated protein 2） and P27^kip1 in bladder transitional cell carcinoma and its clinical significance, Methods：Skp2 and P27^kip1 expressions were investigated in 74 specimens and normal mucous membrane of urinary bladder by SP of immunohisto-chemistry. The different immunoreactivity of Skp2 and P27^kip1 between BTCC and normal mucous membrane was analyzed in relation to the clinical data. It was also analyzed with respect to associations with stage, grade, and prognosis. Results：The positive rates of Skp2 expression and P27^kip1 expression in bladder transitional cell carcinoma were 43.24% and 45.95 %, respectively. Skp2 expression was remarkably higher in BTCC than in normal mucous membrane（ 7.69 % ） and increased with rising grade, but there was no statistically significance between the rates of Skp2 positive expression and tumor clinical stage, Skp2 expression was also higher in recurrence group than in primury group and there was no statistically significance in the other two groups, The positive rates of P27^kip1 expression was lower in BTCC than in normal mucous membrane （76.92% ）, Significant correlation was found between P27^kip1 positive expression and tumor grade and clinical stage, the lower the P27^kip1 positive expression,the higher the tumor grade and clinical stage. In relapsing bladder carcinoma, P27^kip1 positive expression decreased significantly, We did not find the other two groups had statistically significance. There was close inverse correlation between Skp2 expression and P27^kip1 expression and the correlation was statistically significant. Conclusions：Skp2 and P27^kip1 expression may be one of the important prognostic indicators and potentially useful for assessing tumor malignany, and they could be a promising target for future cancer therapy strategies.