骨支架材料修复大鼠颅骨缺损的实验观察

Experimental observation for repair of cranial defects with the two scaffolds.

目的:通过制备大鼠颅骨缺损实验模型,比较β-TCP和Bio-oss在骨组织内的成骨能力及生物降解性,为后续实验提供依据。方法:分别将两种材料植入大鼠颅骨缺损模型,术后4周和8周分批处死动物,硬组织切片,组织形态学观察和高清晰度彩色医学图象分析系统定量测量新生骨百分率和材料降解率,进行统计学分析。结果:Bio-oss组的新生骨百分率高于同期的β-TCP组,而β-TCP组材料降解率高于同期的Bio-Oss组。结论:Bio-Oss的成骨能力相对较强,而β-TCP在体内降解速度较快。

Objective： To investigate which is more suitable for further experiment in the osteogenesis and biodegradafion between the two scaffold materials： Bio-Oss.β-TCP through making the model in rats . Method：Filled the critical size defects which have been created in cranial bone of SD rats with the above scaffold materials. 4 and 8 weeks after operation, the rats were killed and the hard tissue slices were prepared group by group in batches. Then the slices were analyzed with the histomorphology variation and the formation rates of new bone and material biodegradation were measured quantitatively by HMIAS-2000 high definition color clinical image analysis system. The data were analyzed by statistics. Result： Through the analysis of stereomicroscope and the histomorphology, the new bone formation rates of Bio-Oss group is highest in the three groups, the difference is significant （P〈0.05）. the biodegradation of group β-TCP is higher than that of the others, the difference is significant （P〈0.05）.Conclusion： The ability of osteogenesis of Bio-Oss is the greater than β-TCP while the biodegradation of β-TCP is more quickly than Bio-Oss.