碗莲组培快繁技术初探

Preliminary Studies on Shoot Tip in vitro Propagation of Bowl Louts Cultivar

以碗莲‘案头春’茎尖为实验材料,对诱导培养基成份、培养基状态、最佳取材时期以及增殖培养基筛选等方面进行了研究。结果表明:最佳取材季节为10～12月份;碗莲茎尖分化最适宜培养基为MS+1.0mg/L6-BA+0.25mg/LNAA+1.0mg/LGA3;适宜的增殖培养基为MS+2.0mg/L6-BA+0.25mg/LNAA;固液培养状态:上层为5mm厚的MS+1.0mg/L6-BA+0.25mg/LNAA+1.0mg/LGA3液态培养基,下层为1cm厚的MS+1.0mg/L6-BA+0.25mg/LNAA+1.0mg/LGA3固态培养基,更有利于芽诱导;培养过程中污染的芽苗用75%酒精1min、0.1%HgCl210min灭菌效果最好。

the shoot tips of bowl lotus were used as explants for in vitro propagation. The factors affecting the inducibe culture and proliferate mediums and the methods of controlling contamination in culture were studied.The result indicated the suitable timing of taking shoot tips for culture were October to December, the most suitable inducibe medium was 1.0 mg/L 6-BA＋0.25 mg/L NAA＋1.0 mg/L GA3, the best proliferate medium was MS＋4.0 mg/L 6-BA＋0.25 mg/L NAA. the best medium state is liquid-solid state, that is the uper layer’s component is MS＋1.0 mg/L 6-BA＋0.25 mg/L NAA＋1.0 mg/L GA3with 5 mm thickness and the under layer ’s component is MS＋1.0 mg/L 6-BA＋0.25 mg/L NAA＋1.0 mg/L GA3with 1cm thickness.the method of controlling contamination in culture was 75% alcohol 1min and 0.1% HgCl2 10min.