东北四省部分玉米丝黑穗病菌生理分化及RAPD分析

Analysis of Physiological Differentiation and RAPD of Part of Pathogens of S. reilianum

应用鉴别寄主鉴定技术对吉林省、辽宁省、黑龙江省和内蒙古自治区玉米丝黑穗病菌的致病性情况进行了鉴定,共划分为4个不同的致病类型,其中黑龙江省玉米丝黑穗病菌致病性较强,发病率为29.34%,辽宁省玉米丝黑穗病菌致病性较强,发病率为18.00%,说明玉米丝黑穗病菌的致病性存在明显差异。应用RAPD技术对4个省区玉米丝黑穗病菌的生理分化进行了分析,不同菌株的相似系数在0.40～0.64之间,在0.58的相似系数水平上共划分为4个类群,其中505号和506号菌株相似性最大,相似系数达到0.64;504号和507号菌株相似性最小,相似系数达到0.40,说明丝黑穗病菌存在着丰富的生理分化现象。

Pathogens of S. reilianum came from four provinces （Ji lin、Liao ning、Hei long Jiang、Inner Mongolia） were divided into four pathogens types by differential hosts in the field. The pathogens of S. reilianum came from Hei long jiang was maximum, the rate of pathogens was 29.34% and came from Liao ning was minimum, the rate of pathogens was 18.00%. The pathogens of S. reilianum had differentiation. The pathogens of S. reilianum came from 4 provinces were divided into 4 groups by RAPD （percent agreement 0.39）, the percent agreement between 0.40 and 0.64.The biggest percent agreement was 0.64 which came from 505 and 506 pathogens of S. reilianum, the smallest percent agreement was 0.40 which came from 504 and 507 pathogens of S. reilianum. According to the results, pathogens of S. reilianum had the case of physiological differentiation.