应用ATR-FTIR考察壳聚糖及其衍生物对角质层含水量的影响

Study on the Water Content of Stratum Corneum after Treated by Chitosan and Its Derivatives by ATRFTIR

目的:用衰减全反射傅立叶红外光谱考察壳聚糖(CS)及其衍生物对小鼠角质层含水量的影响,以初步探讨其作为透皮吸收促进剂的作用机理。方法:合成不同取代度的N-三甲基壳聚糖(TMC)和N-羧甲基壳聚糖(MCC);酶消化法提取小鼠角质层,分别用2.5%TMC、MCC、CS溶液处理之后进行ATR-FTIR分析,计算酰胺基Ⅰ吸光度和酰胺基Ⅱ吸光度之比。结果:TMC20,TMC40,TMC60,MCC,CS,H_2O(作为对照)处理之后,角质层酰胺基Ⅰ吸光度和酰胺基Ⅱ吸光度之比分别在8,4,8.6,6,6 h达到最大值:1.7286,1.6190,1.6690,1.6460,1.6103,1.5905。结论:壳聚糖及其衍生物可使角质层含水量增加,可能是其具备透皮吸收促进作用的原因之一。

Objective： To study the effect of chitosan （CS） and its derivatives on the water content of stratum corneum by ATR-FT- IR,in order to investigate their mechanisms of enhancing transdermal penetration. Method： N-trimethyl chitosans（TMCs） with different degree of quaternization and N-carboxymethylchitosans （MCCs） were synthesized from chitosan; stratum corneum was extracted from mouse by enzyme digestion, and ATR-FTIR was used to analyze the stratum corneum after treated by chitosan and its derivatives. The ratio of amide Ⅰ band and amide Ⅱ band was calculated. Result： The ration of amide Ⅰ band/amide Ⅱ band reaches a maximum of 1. 7286,1. 6190,1. 6690,1. 6460,1. 6103 and 1. 5905 at 8,4,8,6,6 and 6 h after treated by TMC20, TMC40, TMC60, MCC, CS and H2O,respectively. Conclusion： Chitosan and its derivatives were found to increase the water content of stratum corneum which may be one of the reasons for their penetration enhancing effects.