加减补阳还五汤对佐剂性关节炎大鼠作用的实验研究

Experimental Study on the Effects of Modified Buyang Huanwu Decoction on Adjuvant-induced Arthritis rats

目的研究加减补阳还五汤对大鼠佐剂性关节炎的影响，探讨加减补阳还五汤治疗类风湿性关节炎的作用机制。方法建立大鼠足跖注射弗氏完全佐剂性关节炎模型，观察加减补阳还五汤对动物超敏反应性炎症血清中白细胞介素（IL-1）、肿瘤坏死因子（TNF-α）、循环免疫复合物（CIC）、丙二醛（MDA）含量和超氧化物歧化酶（SOD）活性的影响。结果加减补阳还五汤能降低模型动物的IL-1、TNF-α、CIC、MDA含量，提高SOD活性，均有显著性差异（P〈0．01）。结论加减补阳还五汤具有抗大鼠佐剂性、继发性关节炎作用，其机制与抑制自由基损伤和降低自身免疫的病理生理反应有关。

Objective To study the effects of modified Buyang Huanwu decoction on adjuvant-induced arthritis, and the mechanism of this decoction in treating rheumatoid arthritis. Methods Adjuvant-induced arthritis （AA） model was induced by plantar injection of Freundis complete adjuvant and Observed the level of interlinkin-l（IL-1）, tumor necrosis factor-α （TNF-α）, circulating immune complexes（CIC）, malondialdehyde（MDA） and antioxidizing activity of superoxide dismutase （SOD） in AA rats treated with the decoction. Results The contents of IL-1, TNF-α, CIC, MDA obviously decreased and the activity of SOD was increased by Buyan Huanwu Decoction （P〈0.01） in AA rats. Conclusion Modified Buyang Huanwu decoction shows visibly anti-inflammatory function on AA rats, which is associated with its mechanism of suppressing free radical injure and modifying autoimmune physiological and pathological reaction.