凋亡抑制因子Survivin和Livin在胃癌中的表达及意义

Expression of Survivin and Livin in gastric cancer and its clinical significance

目的:探讨凋亡抑制因子Survivin和Livin在胃癌中的表达和临床病理因素及预后的关系.方法:采用免疫组化SP法检测胃癌组织70例、癌旁组织48例及人胃癌细胞株BGC-823中Survivin及Livin的表达,分析Survivin和Livin的表达与临床病理因素及预后之间的关系.结果:Survivin和Livin在胃癌组织的高表达率显著高于癌旁组织(32.86%vs 2.08%,44.29%vs 4.17%,P<0.01);胞质Survivin和Livin高表达与胃癌的分化程度(P=0.007,P=0.018)及组织学类型有关(P=0.005,P=0.049),胞质Survivin还与胃癌转移有关;胞核Survivin高表达与胃癌的生长方式及远处转移有关,其5年生存率明显低于Survivin核阴性和低表达组(40%vs 66%,P=0.04).结论:Survivin、Livin可作为判断胃癌恶性程度和预后的标志.

AIM： To investigate the expression of Survivin and Livin of apoptosis inhibitors in gastric cancer cells. METHODS： Immunohistochemical staining （S-P method） for Survivin and Livin was performed in 70 gastric cancer tissue samples and 48 adjacent tissue samples and BGC-823 cell, and its relationship with clinical pathological features and prognosis was analyzed. RESULTS： Survivin and Livin were detected both in cytoplasm and in nuclei of gastric cancer cells. The expression of Survivin and Livin was higher in gastric cancer tissue samples than in adjacent tissue samples （32.86% vs 2.08%,44.29% vs 4.17%, P 〈 0.01）. Their expression in cytoplasm was significantly related with the differentiation degree （P = 0.007, P = 0.018） and histological type （P = 0.005, P = 0.049） of gastric cancer. The expression of Survivin in cytoplasm was significantly related with distant metastasis of gastric cancer. The expression of Survivin in nuclei was correlated with the growth pattern and distant metastasis of gastric cancer. The 5 years survival rate of gastric cancer patients having a high nuclear Survivin expression was lower than that of those having a low nuclear Survivin expression （40% vs 66%, P = 0.04）. CONCLUSION： Survivin and Livin are overexpressed in gastric cancer cells. A high Survivin expression in nuclei can serve as an important parameter of prognosis of gastric carcinoma patients.