肾综合征出血热病毒在恙螨体内生存空间和时间的初步研究

Study on Distribution and Life Span of Haemorrhagic Fever with Renal Syndrom Virus in Chigger Mite

目的：检测肾综合征出血热病毒（HFRSV）在恙螨体内的生存空间与时间。方法：将自然界采集的鼠体恙螨幼虫、小黑板采集的游离恙螨幼虫和饲养孵化出的若虫进行石腊包埋切片，应用IFAT检测。结果：发现HFRSV多分布于恙螨的腹部，前体部分较少，个别恙螨前足和中足亦可检测到。取不同时间饲养的恙螨幼虫和饲养孵化出的若虫进行病毒分离，20、80、100和115天后的若虫均分离到HFRSV。结果：该病毒在恙螨体内存在时间较长，并可经期传递。

Chigger mite paraffin section were made from larva on natural mice, free larva and reared nymph and examined by IFAT. The result showed that HFRSV were dispersed on belly of chiggerr mite mostly, and few on front, HFRSV were detected also in front leg and mddle leg in mite. HFRSV were seperated from larva and nyrmph during different time. It showed HFRSV could be attained for the nymph after reared 20, 80, l00, 115 days. It demonstrated that HFRSV could stay in chigger mite for a long time and be transmitted by trans-stadial.