中国兰花(Chinese Cymbidium)部分品种的叶片同工酶分析

CHINESE CYMBIDIUM CULTIVA RCLASSIFICATION WITH LEAF ISOZYME POLYMORPHISM

利用聚丙烯酰胺凝胶电泳和淀粉胶凝胶电泳,对中国兰花的分属于六个种一个变种二十个品种的叶片过氧化物酶、酯酶、酸性磷酸酯酸、多酚氧化酶的同工酶进行了分析,共获得98条酶带,其中的95条具多态性。各个种的品种之间,有基本相似的特征酶谱。而各种间的酶谱差异较大。四种同功酶的聚类分析结果与传统分类学的划分比较接近,为中国兰花品种的鉴定和分类提供了生物化学依据。

Twenty Chinese Cymbidium cultivars from six species and one variety were evaluated for four isozyme systems and cluster analysis was used to examine isozyme divergence among these species. 95 bands of total 98 bands achieved were polymorphic. There were similar patterns within cultivars of the same species; But the patterns between species were diverse. The result of cluster analysis of four kinds of isozyme is similar to the traditional classification. This result provides biochemical data for cultivar identification and classification in Chinese cymbidium.