幽门螺杆菌分离培养的研究

Studies on Isolation and Culture of Helicobacter Pylori

采用细菌培养法及快速尿素酶法分别检测了１６９例消化道疾病患者幽门螺杆菌（Ｈｅｌｉ－ｃｏｂａｃｔｅｒｐｙｌｏｒｉ，ＨＰ）感染情况。标本经２０％葡萄糖运送培养基运送后，分别涂布于选择性培养基和本室改良的选择性培养基，３７℃微氧环境（５％Ｏ２，１０％ＣＯ２，８５％Ｎ２）培养８天。标本同时采用快速尿毒酶法进行检测。结果表明，细菌培养法与尿素酶法检测阳性率接近，分别为４６％和５５％；细菌培养第６天与第８天其阳性检出率相同；选择性培养基与本室改良选择性培养基对幽门螺杆菌的检出率差别明显，分别为３４％（１７／４９）和５１％（６１／１２０），同时其杂菌生长率分别为５４％（２９／４９）和２５％（３０／１２０）。

The positive rate of Helicobacter pylori infcetion in 169 consecutive dyspeptic patients by the methods of urease and culture was described.The positive rate was 55% and 46% respectively.The clinical gastric mucosal biopsy specimens were transported to laboratory within five hours with 20% glucose and then immediately plated on selective culture media(SCM) and improved selective culture media(ISCM) made by the author.The plates were incubated 8 days under microaeropgilic conditions (5%O 2 10%CO 2 85%N 2).The rate of Helicobacter pylori growth on ISCM and SCM was 51%(61/120)and 35%(17/49)separately.The positive rate were identical during 6 or 8 days.The rate of contamination were 15%(30/120)and 59%(29/49) respectively.