五步蛇蛇毒类凝血酶N端的部分氨基酸序列

Partial Amino Acid Sequence of N terminal of Thrombin like Enzyme From Agkistrodon acutus .

从五步蛇蛇毒中纯化得到的类凝血酶 ,在SDS PAGE及IEF均为一条带 ,且分子质量约 38ku ,等电点约为 4 0。测定该酶N端 1 5个氨基酸的序列是VIGGVECDINEHRFL 。

A thrombin like enzyme was purified from the venom of the snake Agkistrodon acutus . It was shown to be a single band both in SDS PAGE and IEF. Its molecular weight is about 38 ku and isoelectric point is near 4 0. Also , its 15 amino acid sequence of N terminal was obtained. It is VIGGVECDINEHRFL. It has high homology with thrombin like enzymes from other snake venoms .