外显子捕捉法快速分离新基因的原理和方法

Principle and Method of Exon Trapping to Isolate Novel Genes Rapidly.

从大片段基因组DNA中快速分离转录序列是疾病基因定位克隆 (positionalcloning)中的关键步骤 .外显子捕捉法是一种较为成功的方法 .文章对该法的基本原理。

Rapidly isolating transcriptional sequences from large fragment genomic DNA is a key step in the positional cloning of disease genes.Exon trapping is one of the most successful methods. The basic principle, methodology improvements and practical achievements of the exon trapping approach are reviewed in detail.