连接介导PCR及其在体内足迹研究中的应用

The Principle of Ligation Mediated PCR and Its Application in in vivo Footprinting Study

连接介导聚合酶链反应是以连接反应为基础的单侧PCR技术 ,首先在DNA片段的一端连接上一个公共连接子 ,而后在这个连接子与另一个DNA序列特异的引物间扩增 .Vent聚合酶的使用 ,延伸产物捕获及连接子标记选择策略的采用 ,大大提高了连接介导聚合酶链反应的敏感性 .

Ligation mediated PCR is a strategy of single sided PCR based on ligation. First, a common linker is linked to one end of DNA fragments, then DNA sequence is amplified between the common linker and a sequence specific primer. The application of vent DNA polymerase and strategies of extension products capture and linker tag selection greatly improves the sensitivity of ligation mediated PCR. The invention of this technique has facilitated the execution of in vivo footprinting study.