C_(60)对体外培养癌细胞的光致作用研究

Studies on the Photoaction Effect of C_( 60) on Cancer Cells in vitro and Its Mechanism

研究结果表明用C60 磷脂酰胆碱与体外培养的HeLa细胞融合后 ,以 4 0 0 0lx光强度激发C60(C60 浓度 2 0mg/L)对HeLa细胞具有显著的光致杀伤作用 .生化测定证实光激发C60 导致膜蛋白巯基含量减少、膜脂过氧化、丙二醛含量增高 ,SDS PAGE证明膜蛋白交联 ,荧光偏振显示膜流动性降低 ,电镜超微膜结构破坏 ,经MTT法检测 ,大部分细胞死亡 .C60 的强烈光致作用证实了Arbogast等认为光激发C60 可产生单线态氧的观态 .

After the fusion between the C 60 phosphatidylcholine liposome (C 60 concentration as 20 mg/L) and HeLa cells and illumination with the Tungsten Halogen lamp (4 000 lx, 30 min ), the most of cells were killed by the evaluation of MTT assay (3 (4,5 dimethythiazol 2 yl) 2,5 diphenyl tetrazolium bromide). Biochemical test showed that photoexcited C 60 led to the decrease of the sulfhydryl content of the membrane protein and the increase of the malonidaldehyde (MDA) with the peroxidation of the membrane lipid. SDS PAGE showed the conjugation of the membrane protein. Fluorescence polarization (FP) indicated that the fluidity of the cell membrane increase.