Nafion膜固定的亚甲基蓝为介体的生物传感器

Hydrogen Peroxide Biosensor Based on Methylene Blue Incorporated into Nafion Membrane as Electron Transfer Mediator

制成了以亚甲基蓝为介体的电流型过氧化氢生物传感器 ,通过离子交换牢固地固定在Nafion膜中的亚甲基蓝 ,能有效地在辣根过氧化物酶和玻碳电极之间传递电子 .探讨了 pH值、温度、工作电位和抗坏血酸等物质对此传感器生物电催化还原H2 O2 的影响 .此生物传感器选择性好、灵敏度高 ,对H2 O2 线性响应范围为 5 0× 1 0 - 7～ 2× 1 0 - 4mol/L ,响应时间少于 30s.

A new amperometic biosensor for hydrogen peroxide based on methylene blue incorporated into Nafion membrane as electron transfer mediator was fabricated. It was found that methylene blue incorporated into Nafion membrane by ion exchanging could effectively transfer electrons between horseradish peroxidase and glassy carbon electrode. Bio electrocatalytic reduction of hydrogen peroxide at the biosensor was evaluated with respect to solution pH, temperature, operating potential and influences of ascorbic acid etc. The biosensor response exhibited fine selectivity, high sensitivity and a linear dependence on the analytic concentration range 5×10 -7 ～2×10 -4 mol/L. Response time was less than 30 s.