阿达玛变换显微图象法测定乳腺癌DNA含量

Measurements of DNA Content in Breast Cancer Cell by Hadamard Transform Microscopic Image Analysis

以吖啶橙为细胞DNA荧光探针 ,用阿达玛 (Hadamard)变换显微图象分析仪和显微荧光光度计分别测定了 4例乳腺肿瘤的细胞DNA含量 (倍性 ) ,并对分析结果进行了比较 ,二者的分析结果均与病理学诊断结论相吻合 .阿达玛变换显微图象分析仪作为一种新的细胞定量分析仪器 ,其分析结果的准确度可与显微荧光光度计相媲美 ,而且阿达玛变换显微图象分析仪还具有其独特的优点 ,如信噪比高。

Using acridine orange as fluorescence probe for DNA in cell, measurements of cellular DNA contents of four cases of human breast tumor were done by the system of Hadamard transform microscopic image analysis and conventional microfluorometry respectively, and the analytical results were compared. The results by the two methods are in agreement and both are concordant with pathological diagnosis. It shows that the new instrument for quantitative cytological analysis Hadamard transform microscopic image analysis system, can provide precise analytical results as that by microfluorometer. In addition, the instrument has outstanding advantages such as high signal to noise ratio, the capability of analyzing two cells or more simultaneously and subtracting background signal synchronously .