GM3与Ca^(2+)-ATP酶的重建及其冷冻断裂电镜观察

The Freeze-fracture Study of Ca^(2+)-ATPase Proteoliposomes Reconstituted with Ganglioside GM3 and Soybean Phospholipids

应用生物膜的分离与重建技术 ,将GM3、大豆磷脂与肌质网Ca2 + ATP酶共同重建在脂质体上 ,酶活力明显增加 .经负染、冷冻断裂复型后电镜等形态学方法证实形成的脂酶体囊泡封闭性好 ,脂酶体上Ca2 + ATP酶蛋白颗粒均匀。

Sarcoplasmic reticulum Ca 2+-ATPase proteoliposomes were reconstituted with ganglioside GM3 and soybean phospholipid by addition of detergent, sonication and chromatography. It was observed that GM3 may enhance the activity of Ca 2+-ATPase in proteoliposomes and increase the diameter of uniform protein particles in sealed-well proteoliposomes vesicles which was evidenced by the negative-staining microscopy and freeze-fracture replica.