期刊文献+

大鼠视交叉上核与室旁核的联系──病毒跨神经元顺行追踪研究 被引量:2

THE CONNECTION BETWEEN THE SUPRACHIASMATIC NUCLEUS AND THE PARAVENTRICULAR NUCLEUS IN THE RAT──STUDY USING ANTEROGRADE OF VIRUS TRANSNEURONAL TRACING
下载PDF
导出
摘要 本实验采用假狂犬病毒(猪疱疹病毒)作为跨神经元追踪示踪物,对下丘脑视交叉上核与下丘脑室旁核的联系进行了研究.实验大鼠于摘除双侧颈上交感神经节后,向单侧眼球内注入假狂犬病毒,术后动物分别存活48、56、72及96h.结果显示:56h组仅在视交叉上核内有极少数的细胞被病毒感染;72h组机交叉上核内被病毒标记的细胞增多,定劳核内也出现了被病毒感染的神经元,主要集中在背内侧帽区;96h组在室旁核内被病毒感染的神经元增加,除背内侧帽区外,其它亚孩也出现了被病毒感染的神经元;各实验组的亚室旁带未见有被病毒标记的神经元.以上表明视交叉上核的传出纤维直接投射到室旁核,两核团神经无间可能存在有直接的突触联系. It is necessary to study the connection of the suprachiasmatic nucleus (SCN) with the rest parts of the brain, since SCN is a dominate pacemaker involved in the generation of circadian rhythm in mamma1s. The paraventricular nucleus (PVN) mainly regulates many of the autonomic and neuroendocrine circadia rhythm, which are controled by SCN. However, the connection between the SCN and PVN is not clear. The present study applied pseudorabies virus(PRV) as an anterograde transsynaptic tracer. Forty adult maIe Sprague-Dawley(SD) rats were subjected to a supracervical sympathetic ganglionectomy to eIiminate viral transport via the sympathetic innervation before uniocular injection of 3μl PRV(Bartha strain,5×108PFU/ml). The rats were killed at 48,56.72 and 96 hours after operation respectivelly. The prefix rinse(100 units ofheparin per 100 ml isotonic saline) and fixative (4. 0% para formaldehyde, 0. 25% g1utaraldebyde, 1. 37% DL-lysine and 0.214 % sodium-m-periodate in 0. 1 mol/L PB pH 7. 4) were used. The brain was removed from the skull and stored in the fix ative for 1 h, then transferred into 20% sucrose in 0. 1 mol/L PB(pH 7. 4) for 24 hours. Neurons labeIled with PRV were shown by immunofluorescent and immunohistochernical ABC methods. Results: (1)No neuron was infected by PRV in the SCN and the PVN at 48 hours. (2)A few neurons only in the SCN were infected by PRV at 56 hours. (3)Numerous neurons labelled by PRV presented in SCN, but in the PVN the labelling neurons were found predominatly in the dorsomedial cap at72 hours. (4)With the survival time, the number of neurons infected by PRV were increasing, PRV labelling neurons occutred in the medial patvocellular part and lateral magnocellular part of the PVN besides in the dorsomedial cap at 96 hours.However, no neuron labelled by PRV was found in the subparaventricular zone. This study indicates that there is a directprojection frorn SCN to the PVN and PRV is a useful transneuronal tracer.
出处 《神经解剖学杂志》 CAS CSCD 北大核心 1997年第1期11-14,共4页 Chinese Journal of Neuroanatomy
关键词 视交叉上核 假狂犬病毒 跨神经元追踪 室旁核 pseudorabies virus, transneuronal tracing, suprachiasmatic nucleus, paraventricular nucleus, rat
  • 相关文献

同被引文献12

  • 1童建,朱金华.免疫系统的昼夜生理节律[J].免疫学杂志,1995,11(2):99-101. 被引量:10
  • 2Blalock J E:A molecular basis for bidirectional ommunication between the immune and neuroendocrine systems. Physiol Rev. 1998,69( 1 ): 1-32.
  • 3Ishizuka Y, Ishida Y,Jin QH, et al. Abdominal vagotomy atenuates interleukin-1β-induced nitric oxide release in the paraventricular nucleus region in conscious rats. Brain Research, 1998, 789 ( 1 ): 157-161.
  • 4Costa A, Trainer P, Besssr M, et al: Nitric oxide modulates the release of cortico-traphin-releasasion hormone from the rat hypothalamus in vivo. Brain Res,1993,605( 1 ) : 187-192.
  • 5Ivier C. Blockade of nitric oxide formation augments adrenocorticotropin release by blood-borne interleukin- 1β: Role of vasopressin, prostaglandins, and aladrenergic receptors. Endocrinology, 1995, 136 ( 8 ):3597-3603.
  • 6Hron J, Smith PM. Nitric oxide actions in paraventricular: cardiovascular and neurochemical implications. Am J Physiol, 1994 266(1-t2) :306-313.
  • 7Zabel P, Linnemann K, Schlaak M:Circadin rhythm in cytokines. Immun Infect, 1993, 21 ( Suppl1 ): 38-40.
  • 8Petrovsk N, Mcnair P,Harrison LC. Diurnal rhythms of pro-inflammatory cytokines: regulatuon by plasma cortisol and therapeutic implications. Cytokine, 1998,10(4) :307-312.
  • 9杨宏,邱建勇,鞠躬.GAP-43免疫反应神经纤维对发育中大鼠脾脏支配的研究[J].神经解剖学杂志,1997,13(1):1-5. 被引量:1
  • 10娄雪林,金必连,余承高,吴鸿修,周专.内源性一氧化氮在下丘脑室旁核的心血管作用[J].同济医科大学学报,1999,28(6):500-502. 被引量:4

引证文献2

二级引证文献3

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部