大鼠视网膜缺血后Parvalbumin免疫反应神经元的变化

VARIATIONS OF THE PARVALBUMIN IMMUNOREACTIVE NEURONS IN ISCHEMIC RETINA OF RAT

本文观察了大鼠视网膜缺血后Parvalbumin（PV）免疫反应神经元的变化。动物分为缺血10min组、15min组、30min组及60min组等4组．动物右眼为缺血眼，左眼做自身对照眼．结果表明PV免疫反应神经元主要位于内核层及节细胞层，其突起伸向内网层第1、5亚层，神经纤维层也可见PV免疫反应纤维。缺血10min后PV免疫反应神经元未出现变化，缺血15min后数量开始减少，内网层第5亚层PV免疫反应纤维消失、缺血30min、60min后PV免疫反应神经元比缺血15min后减少明显．表明缺血15min后即出现PV免疫反应神经元的变化，但各缺血时间点上其减少率低于其它类型的神经元，提示它对缺血有一定的耐受性。

The variation of parvalbumin immunoreactive (PV-I)neurons in the ischernic retina of SD rat was studied. The experimental animals treated with ischemia of different time were divided into four groups (l0' l5, 30 and 60 min group).The left eye of rats was seIf control eye and right eye was ischemic eye. The results showed that PV-I neurons were located in inner nuclear layer (INL) and ganglion cell layer (GCL), their processes extended toward inner plexiform layer (IPL) and were distributed in 1, 5 sublarninaeof IPL. PV-I fiber could be seen in nerve fiber layer. There was no significant change in 10 min group. The density of PV-I neurons were decreased and the PV-I fibers in 5 sublamina of IPL were lost after l5 min of ischemic treatment. The density of PV-I neurons in 30 min group and 60 rnin group was decreased more clearly than that of 15 min group. The results suggested that decrease 0f PV-I neurons may occur after 15 min ischemia but the decreasing rate was lower than that of other neurons. It is indicated that PV-I neurons have some tolerance capacity to ischemia.