血清中磺草酮的液相色谱测定法

Determination of Sulcotrione Herbicide in Serum by HPLC

目的建立液相色谱法测定血清中新型除草剂磺草酮的方法。方法以甲醇和0.1mol/L磷酸二氢钠三乙胺缓冲液作为流动相,254nm检测波长进行样品测定。结果回归方程:Y=20742.17X+655.12(r=0.9993),检出限:0.044mg/L,样品分析的RSD在4.83%～6.79%之间,回收率在94.65%～109.23%之间。结论该方法具有较高的准确度和精密度,而且简便快速,具有较高的实际应用价值。

[ Objective ] To establish HPLC method for the determination of sulcotrione herbicide in serum. [ Methods ] Methanol and 0. 1 mol/L triethylamine sodium dihydrogen phosphate were applied for the mobile phase; the detection was conducted with the wavelength of 254nm. [ Results] The regression equation was Y = 20742. 17 X ＋ 655. 12 （ r = 0. 9993 ）, the detection limit was 0. 044 mg/L, the RSD was between 4.83% and 6.79% ; the recovery rate was 94.65% - 109.23%. [ Conclusion] This method is simple and rapid with high accuracy, precision and application value.