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类法尼醇X受体在巨噬细胞中下调单核细胞趋化蛋白1的表达 被引量：1

Downregulation of Monocyte Chemoattractant Protein-1 by Farnesoid X Receptor in Murine Macrophages
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摘要 目的研究小鼠巨噬细胞株ANA-1中类法尼醇X受体的表达情况,并进一步研究类法尼醇X受体天然配体鹅脱氧胆酸在ANA-1中对单核细胞趋化蛋白1表达的影响,以探讨类法尼醇X受体在动脉粥样硬化发生发展中的作用。方法应用逆转录聚合酶链反应检测类法尼醇X受体在ANA-1细胞中的表达,及鹅脱氧胆酸处理后单核细胞趋化蛋白1的转录水平,Westernblot检测类法尼醇X受体的表达及其激活后对自身蛋白表达的调节,以及单核细胞趋化蛋白1蛋白的表达水平。结果类法尼醇X受体在ANA-1中表达,并依赖鹅脱氧胆酸浓度自我上调,鹅脱氧胆酸能显著下调单核细胞趋化蛋白1的表达水平(P<0.05)。结论类法尼醇X受体在小鼠巨噬细胞株ANA-1中组成型表达并呈配体浓度依赖性自我上调,类法尼醇X受体激活后可通过直接或间接方式下调单核细胞趋化蛋白1的表达。 Aim To investigate expression of famesoid X receptor （FXR） in murine macrophage cell line ANA-1 and evaluate the effect of FXR agonist ehenodexyeholie acid （CI）CA） on the expression of monocyte ehemoattractant protein- 1 （MCP- 1 ） in ANA-1 cells. Methods ANA-1 cells were cultured and treated with FXR agonist CI）CA. The mRNA level of FXR and MCP-1 were detected by reverse transcription-polymerase chain reaction （RT-PCR）. Western blot was used to assess the expression of FXR and MCP-1 protein level. Results FXR expressed in ANA-1 cells and acted in an autoregulatory fashion, CD- CA significantly reduced MCP- 1 mRNA and protein level in ANA-1 cells （ P 〈 0.05）. Conclusions FXR experss in murine macrophage cell line ANA-1. After activated by CDCA, FXR may downregulate MCP-1 directly or indirectly.
作者 张迁 甘淋 鲁力 申丽丽 何凤田 江渝
机构地区 中国人民解放军第三军医大学基础医学部生物化学与分子生物学教研室
出处 《中国动脉硬化杂志》 CAS CSCD 2008年第1期21-24,共4页 Chinese Journal of Arteriosclerosis
基金 第三军医大学留学回国人员启动基金(06HG010) 重庆市自然科学基金(CSTC 2007BB5050)
关键词 分子生物学 类法尼醇x受体 核受体 胆汁酸 单核细胞趋化蛋白1 ANA-1细胞株 动脉粥样硬化 Farnesoid X Receptor Nuclear Receptor Chenodexycholic Acid Monocyte Chemoattractant Protein-1 Macrophage Atherosderosis
分类号 Q7 [生物学—分子生物学]
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参考文献20

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共引文献7

同被引文献8

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二级引证文献3

中国动脉硬化杂志
2008年 第1期

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