血管外膜剥离致内膜增生病变的动物模型

A New Animal Model:Removal of Adventitia Induced Neointimal Development

目的建立颈动脉外膜剥离引起内膜增生的动物模型,为研究血管外膜在动脉粥样硬化发生中的作用奠定基础。方法2g/L的Ⅱ型胶原酶消化颈动脉外膜后,眼科镊钝性剥离外膜,HE染色明确外膜剥离效果;免疫组织化学染色观察外膜剥离对内膜的影响。结果酶化学消化+眼科镊钝性剥离可有效剥离血管外膜;外膜剥离2周后可见相应内膜处出现增生性病变,病变成分为分泌型平滑肌细胞;而外膜保留侧正常。结论胶原酶消化+眼科镊钝性剥离的方法可有效剥离血管外膜,促进血管内膜增生性病变形成。

Aim To develop a new animal model for the investigation of adventitial function in atherosclerosis. Methods One carotid artery adventitia of rabbit with 2 mg/mL typeⅡ collagenase was digested, then adventitia with microforeeps was removed, and the contralateral carotid artery was used as control. HE staining and immunohistochemistry were performed to assure adventitia was removed, and to assess intimal change after adventitial removal. Results It is feasible to remove vascular adventitia with collagenase digestion and mechanical dissection. Removal of adventitia of rabbit carotid artery induced neointima development in 2 weeks. Cells in neointima were secreating smooth muscle cells. The other sides were normal. Conclusions The method we used to remove artery adventitia is effective. Normal adventitia is necessary for normal vessel structure.