非小细胞肺癌组织中表皮生长因子受体与磷酸化Akt蛋白的表达及其意义

Epidermal growth factor receptor and phophorylated Akt protein expression in non-small-cell lung cancer

目的评价非小细胞肺癌（NSCLC）标本中表皮生长因子受体（EGFR）与其信号传导通路下游的磷酸化Akt（p-Akt）蛋白质表达的意义。方法制作189例非小细胞肺癌标本的组织微阵列，运用免疫组织化学方法检测肺癌组织中的EGFR和p-Akt蛋白质的表达，分析该两种蛋白表达与患者预后生存之间的关系。结果EGFR在NSCLC中的阳性表达率为54．5％（103／189），单因素分析提示其表达程度与性别（χ^2=4．267，P=0．039），病理类型（χ^2=14．749，P=0．002），分化程度（χ^2=6．295，P=0.043）以及病理分期（χ^2=9．318，P=0．025）相关，而Logistic多因素回归分析显示EGFR表达仅与病理类型之间的相关性具有统计学意义（P=0．047）。p-Akt在NSCLC中的阳性表达率为51．3％（97／189），其阳性表达率与各临床病理特征无关（P〉0．05）。二者阳性表达均与预后无关（P值分别为0．972和0．903）。结论NSCLC中EGFR和p-Akt蛋白质阳性表达率对患者预后无提示意义，尚不能以EGFR或p-Akt蛋白质表达作为NSCLC分子分期或判断预后的指标。

Objective To evaluate the expressions of epidermal growth factor receptor （EGFR） and phosphorylated Akt （p-Akt） in non-small-cell lung cancer （NSCLC）. Methods 189 NSCLC samples were made into tissue microarray and immunohistochemistry was used to detect the expression of EGFR and p-Akt proteins. The association of the expressions of EGFR and p-Akt protein with the survival time was evaluated. Results The EGFR and p-Akt protein positive rates were 54. 5% and 51.3% respectively. Univariate analysis showed that the EGFR expression was associated with gender（χ^2= 4. 267, P = 0. 039 ）, histology（χ^2 = 14. 749, P = 0. 002 ）, differentiation （χ^2 = 6. 295, P = 0. 043 ）, and pathological stage （χ^2 = 9. 318, P = 0. 025 ）. Logistic multivariate analysis showed that only the pathological type was associated with EGFR expression（ P = 0. 047 ）. The p-Akt expression was not related with the above clinicopathological features（ P 〉0. 05 ）Kaplan-Meire survival analysis showed that neither of the two proteins had an impact on the patients＇ survival （P = 0. 972 and P = 0. 903 respectively）. Conclusion Protein expressions of IEGFR and p-Akt have no impact on NSCLC patients＇ survival, thus so far they can not serve as molecular staging or prognostic indicators.