自体移植冻存骨髓基质细胞修复犬牙周缺损的实验

Effect of cryopreservation of bone marrow stromal cells on periodontal tissue regeneration

目的观察冻存对骨髓基质细胞（bone marrow stromal cells，BMSC）体内形成牙周支持组织能力的影响。方法在5只Beagle犬的26个实验牙位制备牙周缺损。区组随机法分成3组，空白组（8颗）植入载体胶原膜，未冻存组（9颗）和冻存组（9颗）接种未冻存或冻存细胞与胶原膜复合物，8周后观察牙周组织再生情况。结果冻存组和未冻存组牙槽骨再生百分比分别为（83．7±10．6）％、（85．0±6．8）％，两组新生牙骨质和牙周膜百分比均为100％，差异无统计学意义（P〉0．05）；空白组牙槽骨再生百分比为（24．7±9．2）％，牙骨质再生百分比为（21．0±4．8）％，牙周膜再生百分比为（24．8±5．4）％，均较冻存组和未冻存组显著减少，差异有统计学意义（P〈0．05）。结论冻存对BMSC体内分化形成牙周组织的能力无显著影响。

Objective To investigate the influence of cryopreservation on bone marrow stromal cells＇（BMSC） capability of enhancing periodontal regeneration. Methods Twenty-six artificial periodontal defects were established in 5 Beagle dogs and divided into 3 groups at random. Only collagen membrane, the complex of cryopreserved and uncryopreserved BMSC and collagen scaffold were transplanted in the blank control group, cropreserved and uncryopreserved groups respectively. The periodontal regeneration was observed 8 weeks after transplantation. Results The percentage of periodontal regeneration in cryopreserved and uncryopreserved groups was significantly greater than that of the blank control group （ P 〈 0. 05 ） , but no statistical difference was found between cryopreserved and uncryopreserved groups. Conclusions Cryopreservation had no significant negative effects on BMSC capability of enhancing periodontal regeneration.