胆道梗阻解除术后肾水通道蛋白2表达变化的实验研究

An experimental research of the changes of AQP2 expression after relieving of the obstruction of the biliary tract

目的明确水通道蛋白2(aquaporin 2 protein,AQP2)在梗阻性黄疸梗阻解除术后大鼠肾脏的表达情况。方法采用蛋白质印迹(western blotting)法及RT-PCR方法检测在梗阻性黄疸梗阻解除后不同时间的大鼠肾脏AQP2及其mRNA的水平。结果AQP2蛋白水平在0h,24h,72h随时间推移逐渐降低,1周时明显高于解除梗阻0h的水平,但所有实验组均较正常对照组(假手术组)低(P<0.05)。而AQP2的mRNA水平在梗阻性黄疸梗阻解除后0h,24h,72h随时间推移逐渐性升高,1周时回落,但比0h水平还要高,然而所有实验组均较正常对照组(假手术组)高(P<0.05)。结论梗阻性黄疸梗阻解除术后肾功能在早期受损逐渐加重,AQP2蛋白水平下调,而AQP2基因水平上调。1周时这些结果仍未恢复正常。AQP2在梗阻性黄疸梗阻解除术后肾功能的变化上可能起到了重要的作用。

Objective To study the expression of aquaporin 2 protein （AQP2） in the kidney after relieving of the obstruction of the biliary tract. Methods Western blotting and RT-PCR were used to determine the protein and mRNA level of AQP2 in different time after the relieving of the obstruction of the obstructive jaundice. Results Within 72 hours of post-released obstructive jaundice, the mRNA level of AQP2 was increased gradually, while its protein level was gradually decreased with the progress of duration at 0 hour, 24 and 72 hours. One week after release, the mRNA level was decreased but still higher than that of 0-hour group. Correspondingly, the protein level was increased and even higher than that of 0-hour group. The protein level of all treated groups was lower than that of normal group （false operation group）, in contrast, the mRNA level was higher. Conclusion The study implies that in the first few days of post-released obstructive jaundice model in rats, the renal function becomes worse gradually, with protein and mRNA level of AQP2 decreased and increased respectively. They do not recover during one week. It implies that AQP2 may play a great role in the recovery of the renal function of post-released obstructive jaundice model in rats.