乳腺癌中的p53基因mRNA高表达的研究

Overexpression of p53 Gene mRNA in Breast Cancer

应用反转录-半定量PCR技术检测47例乳腺癌组织中p53基因mRNA表达，并与临床预后因素进行统计学分析。结果发现：在乳腺正常腺体中有稳定的p53mRNA中度表达，而在乳腺癌组织中，p53mRNA呈高表达者为40．4％（19／47），且以在ER阴性的乳腺癌中增高明显（P＜0．05）。本文提示：p53基因在人类乳腺癌形成和肿瘤演进过程中起重要的作用，p53mRNA高表达的病人浸润性强、预后差。反转录一半定量PCR技术具有快速、简单、敏感可适于临床大规模应用之优点。

p53 gene mRNA expression was examined in 47 cases of breastcancer by using RT/PCR technique, and the results were compared with the clinicalparameters. In normal breast glandular tissue there was moderate p53 mRNA expression, but in cancer tissues, 40. 4% (19/47) of the instances had p53 mRNAoverexpression and the positive rate was significantly high in ER negative cases (P<0. 05). The data indicate that p53 gene may play an important role in the occurrence and progression of breast cancer. The RT/PCR technique is a quick, simpleand sensitive method and suitable for broader clinical use.