林可霉素发酵液膜分离工艺条件的研究

Study on membrane-extraction separation processes of lincomycin fermentation fluid

对影响林可霉素发酵液膜分离性能的主要工艺参数及对后序萃取工艺的影响进行了研究，确定的最佳膜分离工艺为：采用螺旋卷式膜组件，进料温度控制在30℃以下；超滤压力0．2MPa、纳滤压力1．0MPa；超滤膜面流速300L／11，纳滤膜面流速90L／h；超滤浓缩倍数3．0，纳滤浓缩倍数3.0～4．0之间效果最好．林可霉素板框滤液经超滤、纳滤后再萃取，可使萃取级数由9级减少到4级，混合醇用量减少到原来的1／3，林可霉素收率比原来提高8．9％．

The membrane- extraction separation process of lincomycin fermentation fluid was studied in this paper. The effect of main operation parameters on membrane separation performance between protein and Lincomycin and the extraction efficiency were investigated. When spiral module of UF and NF was used for treating Lin-frame filtrate, the optimum operation condition is： feed temperature below 30 degrees, UF pressure 0.2 MPa, NF pressure 1.0 MPa; uhrafiltration volume flow 300 L/h, Nanofiltration volume flow 90 L/h; ultrafiltration concentration multiples 3.0, Nanofiltration concentration multiples between 3.0--4.0. The extraction series will decrease from 9 to 4, mixed alcohol consumption decreased to one- third of that with general process, the recovery of Lincomycin was 8.9% higher than that with general process.