原发性肝癌中丙型肝炎病毒感染及其基因型

PREVALENCE AND GENOTYPS OF HCV INFECTION IN PATIENTS WITH PRIMARY HEPATOCELLULAR CARCINOMA

应用酶联免疫法（EIA）及聚合酶链反应技术（PCR）对98例原发性肝癌患者血清丙型肝炎病毒抗体（抗-HCV）和丙型肝炎病毒核糖核酸（HCVRNA）进行检测，并对HCV感染者应用多引物分型RPCR法进行基因分型。结果显示HCV总的感染率为16．3％（16例），明显高于慢性肝炎组的9．1％及健康对照组的4．5％（P均＜0．05），其中68．8％（11例）为与HBV双重感染，31．2％（5例）为HCV单一感染。对以上HCV感染者进行基因分型，结果Ⅱ型占93．8％，Ⅱ／Ⅲ型混合感染占6．2％。研究表明广州地区原发性肝癌的发生与HCV感染有关，但与HCV、HBV重叠感染的关系较与HCV单一感染的关系更大。HCV－Ⅱ型感染在HCV相关肝癌的发生中起主导作用。

Anti -HCV and HCV RNA were detected in the serafrom 98 patients with primary hepatocellular carcinoma (PHC). The meults showed tha the prevalence of HCV in-fection (Anti - HCV and/or HCV RNA positive) was 16.3% (16/98) which was significantly higher than that in pa-tients with chronic hepatitis (9.1%,30/328) and inhealthy controls (4.5%,3/67). Of the 16 HCV -infectedPHC patients, 11 (68. % ) were HCV/HBV coinfection,the other 5 (31. 2% ) wer HCV single infection. Also,thesera from the above HCV -infected patients were typed byPCR with core region type- specific primers.Tt was foundthat93. 8% (15/16) were infected with HCV type Ⅱ,6.2% (1/16) were infeded with tpye Ⅱ & Ⅲ。 Our re-search indicates tha the occurence of PHC in Guangzhouareas is related to HCV infectign, espeially to HCV/HBVcoinfection, and tha HCV -Ⅱ plays a predominant role inHCV - related PHC.