N^1-乙基-N^11-(环庚烷基)甲基-4，8-二氮杂癸烷对宫颈癌细胞的作用

Effect of N^1-ethyl-N^(11)-(cycloheptyl)methyl-4,8-diazaundecane on cervical cancer cells

目的探讨N1-乙基-N11-(环庚烷基)甲基-4,8-二氮杂癸烷(CHEN)对宫颈癌细胞株Siha的抗肿瘤作用。方法MTT法检测细胞的生长情况;流式细胞术及DNA片段化分析法检测细胞凋亡;化学分析法测定精胺氧化酶(SMO)活性。结果CHEN可显著抑制Siha宫颈癌细胞生长,且抑制作用随药物浓度增加而增强,用10μmol·L-1 CHEN处理细胞,24和48h生长抑制率分别高达61%和75%。细胞凋亡分析发现,CHEN处理可诱导Siha细胞凋亡,导致凋亡峰出现和细胞核DNA片段化。在0~20μmol·L-1浓度范围内,CHEN作用24h对Siha细胞SMO活性无明显影响。结论CHEN通过诱导细胞凋亡而抑制宫颈癌细胞生长,该抑制作用与SMO活性无关。

AIM To study the anti-tumor effects of N^1 -ethyl-N^11 --（ cycloheptyl ） methyl- 4,8-diazaundecane （CHEN） on the Siha hu- man cervical cancer cells. METHODS MTT was used to assay the cell survival. How cytometry and DNA fragmentation assays were used to determine the cell apoptosis. Chemical analysis method was used to determine the enzyme activity of spermine oxidase. RESULTS CHEN greatly inhibited the cell growth of Siha cancer cells in a concentration-dependent manner. The treatment with 10 μmol. L-1 CHEN for 24 and 48 h resulted in an inhibitory rate of 61% and 75%, respectively. Apoptotic assay indicated that CHEN Could induce apoptosis of Siha cells and result in appearance of sub-G1 peak and＂ DNA fragmentation. No significant effect on spermine oxidase activity in Siha cells was observed when the cells were treated with 0-20 μmol. L-1 CHEN for 24 h. CONCLUSION CHEN inhibits survival of Siha cervical cancer cells by inducing apoptosis and this inhibition is not related to the activity of spermine oxidase.