乙醇对嗜酒和非嗜酒小鼠体重和肝脏组织形态的影响

Effects of ethanol intake on body weight and liver tissue morphology in alcohol-preferring and alcohol-non-preferring mice

目的比较乙醇对嗜酒和非嗜酒小鼠躯体损害的易感性。方法以近交系C57BL/6J小鼠(嗜酒者)和远交系ICR小鼠(非嗜酒者)为实验动物,采用强制性饮酒程序:饮酒组小鼠饮用乙醇溶液8d,乙醇浓度从3%递增至20%。每天检测小鼠体重、摄食量、饮液量和乙醇摄入量。饮用乙醇期间取小鼠肝脏用组织化学方法观察肝脏组织病理学变化。结果乙醇对嗜酒小鼠体重增长的抑制作用明显强于非嗜酒小鼠。乙醇对两组小鼠的摄食量均有抑制作用,抑制程度无显著差异。乙醇抑制非嗜酒小鼠的饮液量,但对嗜酒小鼠无影响。实验期间两组小鼠的饮酒量相当,但嗜酒小鼠的肝脏损伤更为严重。结论嗜酒小鼠对乙醇毒性作用的耐受性弱于非嗜酒小鼠。

AIM To compare the vulnerability to alcohol-induced physical damage in alcohol-preferring and alcohol-non-preferring mice. METHODS Forced ethanol intake paradigm was carried out with the inbred C57BL/6J mouse strain （alcohol drinker） and with the ICR （non-alcohol drinker） mouse strain used as a outbreed control strain. In ethanol group, C57BL/6J or ICR mice were fed with single bottle containing ethanol liquid with different concentration increasing from 3% to 20% for 8 consecutive days. Body weight, and food, liquid and ethanol consumption in C57BL/6J and ICR mice were observed and measured each day. Further more, histological hepatic changes in forced ethanol intake paradigm were microscopically evaluated. RESULTS In ethanol groups, body weight gain was suppressed more significantly in C57BL/6J mice than in ICR mice. Forced ethanol intake reduced food consumption in ICR mice as well as in C57BL/6J mice to the same extent. It inhibited liquid consumption in ICR mice, but not in C57BL/6J mice. In forced ethanol intake paradigm, C57BL/6J and ICR mice were not significantly different in their ethanol consumption, but histological evaluation indicated that the hepatic lesions were more severe in C57BL/6J mice. CONCLUSION Alcohol-preferring mice may be of poorer tolerance to ethanol toxic effects than alcohol-non-preferring mice.