肿瘤坏死因子-α基因修饰口腔鳞癌DNL体外抗肿瘤的实验研究

Experimental study on antitumor effect in vitro of modified DNL of oral squamous cell carcinoma with TNF α gene

实验目的是观察肿瘤坏死因子－α在体外抗肿瘤的活性，探讨其治疗口腔癌的可行性。通过缺陷型逆转录病毒介导，将肿瘤坏死因子－α基因导入体外培养的人口腔鳞癌引流区淋巴结淋巴细胞（ＤＮＬ），经ＰＣＲ技术、ＴＮＦ－α活性检测技术及Ｇ４１８培养基筛选试验证实外源基因确已导入ＤＮＬ中，并能够表达。应用ＭＴＴ比色法比较转导及未转导基因的ＤＮＬ抗人舌鳞癌Ｔｃａ８１１３细胞的活性。结果显示，转导ＴＮＦ－α基因ＤＮＬ的细胞毒活性明显强于未转导基因ＤＮＬ。

The gene coding for TNF α was introduced into DNL of human oral squamous carcinoma by retroviral mediated gene transduction, and experiments proved that the gene had been introduced into the DNL and the DNL could secrete TNF α of high activity with PCR analysis, bioactivity analysis of TNF α, selection test in G418. Cytotoxicity of gene transduced and nontransduced DNL to Tca8113 cell was compared with MTT method. The result showed that cytotoxicity of the former was higher than that of the latter.