蛋白激酶C抑制剂灯盏花素对低氧预适应小鼠海马NO/cGMP信号转导系统的影响

Effect of protein kinase C inhibitor,erigeron breviscapus,on NO/cGMP signaling pathway in hippocampus of hypoxic preconditioning mice

目的探讨NO/cGMP信号转导系统在低氧预适应中的作用及灯盏花素对其的影响。方法将B lb/c近交系小鼠随机分为空白对照组(H0组)、低氧对照组(H1组)、低氧预适应组(H4组)、药物对照组(C)和灯盏花素组(EB)。观察H1、H4、C和EB组小鼠低氧耐受时间;用分光光度计法测定各组小鼠海马NOS活性、NO产量和放射免疫法测定cGMP含量。结果(1)H4、C和EB组小鼠低氧耐受时间显著多于H1组,且EB组明显多于H4组,H4和C组没有显著差异。(2)H1、H4、C和EB组NOS活性、NO、cGMP含量均高于H0组,H4、C和EB组明显低于H1组,且EB组显著低于H4组,H4和C组没有明显差异。结论NO/cGMP信号转导系统在低氧预适应中起重要作用,灯盏花素通过抑制NO/cGMP信号转导系统参与低氧预适应的产生。

Objective： To explore the role of NO/cGMP signaling pathway in hypoxic preconditioning and the effct of erigeron breviscapus on it.Methods： Blb/c mice were randomly divided into five groups： the normal control group（H0）,the hypoxic control group（H1） and the hypoxic preconditioning group（H4）,the drug control group（C） and the erigeron breviscapus group（EB）.Hypoxia tolerance time of H1,H4,C and EB group were observed.NOS activity and NO production of hippocampus were measured by spectrophotometry and its cGMP content by radioimmunossay.Results：（1） Tolerance time of H4,C and EB group increased significantly than that of H1 group,and the time of EB group even increased than that of H4 group.There was no difference between H4 and C group.（2） NOS activity,NO production and cGMP content of hippocampus were significantly increased in H1,H4,C and EB group as compared with those of group H0.NOS activity,and were significantly decreased in H4,C and EB group than those of group H1,and were significantly decreased of EB group than those of H4 group.There was no difference between H4 and C group.Conclusion： NO/cGMP signaling pathway may play important role in hypoxic preconditioning.Erigeron breviscapus can involve in mechanism of hypoxic preconditioning by inhibiting the activity of NO/cGMP signaling pathway.