成人牙周炎龈组织内浆细胞的超微结构观察

Ultrastructural morphological observation on the plasma cells in periodontitis affected human gingival tissue

为了探讨牙周炎龈组织的免疫反应机理，对２０例牙周炎龈组织进行了透射电镜观察。结果表明，在炎性浸润细胞中以浆细胞（ｐｌａｓｍａｃｅｌ，ＰＣ）数目最多且有变性，根据浆细胞胞核、质膜及粗面内质网腔的变性程度，将浆细胞分为４期。浆细胞核中染色质从正常形态逐渐趋于浓聚、边集、固缩和破碎，质膜从完整清晰、部分缺失到全部丧失其完整性；粗面内质网腔从狭窄、扩张、断裂至囊泡化，其腔内容物的电子密度由低至高再降低；卢氏体（Ｒｕｓｓｅｌ′ｓｂｏｄｉｅｓ，Ｒｂ）从偶见到数目增多再减少；变性浆细胞最终发生凋亡。我们的观察结果提示，成人牙周炎龈组织反应有体液免疫参与。

The transmission electron microscope was used to demonstrate the mechanism of immunoreaction in periodontitis affected gingival tissue. The result showed that the number of plasma cells (PC) was the highest in infiltrated inflammatory cells and with degeneration. According to the variation of ultrastructural features of nuclei; plasma membrane (PM) and cisternae (Ci) of rough endoplasmic reticulum (RER), PC were classified into 4 stages. The chromatin of their nuclei varied from normal, to marginal concentrated, pyknosis, and karyorrhexis. The PM appeared intact, partial loss, to complete loss. The Cisternae showed varying degree of dilatation and the RER was broken into numerous discrete vesicles. The electron density of cisternae varied from low to high, and again low.The number of Russell′s bodies varied from rare to increase, and then rare again. At last degenerated PC underwent apoptosis. The results show that the adult periodontitis is related to humoral immunity.