摘要
目的探讨晶体混浊过程中晶体蛋白成分及其结构变化。方法首次用FTRaman和FTIR(FouriertransformRamanandinfrared)光谱及利用相关峰积分的半定量方法对老年性白内障不同混浊程度的晶体构像变化进行研究。结果按Chylack等的LOCS方法划分为Ⅲ级晶体的两种光谱特性介于0级和Ⅳ或Ⅴ级晶体之间;Ⅳ和Ⅴ级晶体的两种光谱构像非常相似,它们的一些谱峰与0级晶体的有明显区别。两种光谱对它们的检测既有一致性,也有互补性。结论晶体混浊发展不仅与老年性白内障晶体的三种晶体蛋白含量有关,也与其晶体蛋白中的SH、trp、phe和-CH2等受到各种损伤有关。
Objective In order to search for the main composition and conformational changes of lens proteins in senile cataract. Methods We firstly used Fourier transform Raman and infrared (FTRaman and FTIR) spectroscopy and semi quantitative method in the integral calculus of related peaks to determine the conformational changes of lens protein during opacification of varying grade. Results The spectral features of lenses with grade Ⅲ cataract were intermediate between human clear lenses (grade 0) and grade Ⅳ or grade Ⅴ cataractous lenses. The spectral characteristics of lenses with grade Ⅳ were very similar to that with grade Ⅴ lenses, and some peaks of them were not seen in human clear lenses. The two spectral analyses showed that some findings were identical and some findings were complementary. Conclusion The development of lens opacification is correlated not only with the relative contents of α ,β ,γ crystallines in lens proteins but also with the various damaged SH, trp, phe, -CH 2 in lens protein of senile cataract.
出处
《中华眼科杂志》
CAS
CSCD
北大核心
1997年第5期337-339,共3页
Chinese Journal of Ophthalmology
基金
北京大学稀土材料化学应用国家重点实验室基金