紫外诱变对绿僵菌素生产的影响

Effects of UV Mutation on Destruxins Yields of Metarhizium anisopliae

利用金龟子绿僵菌Metarhizium anisopliae var. ansopliae MaQ10菌株作为出发菌株,应用紫外线(UV)诱变其分生孢子和原生质体,分别测定了40个突变株的绿僵菌素产量.结果表明,出发菌株MaQ10的绿僵菌素A和B(DA和DB)产量分别为(106.81±9.55)和(29.53±2.70)μg/mL,分生孢子突变株的DA和DB平均产量分别为(45.05±7.77)和(10.83±1.05)μg/mL,原生质体突变株的DA和DB平均产量分别为(68.97±6.07)和(11.80±1.35)μg/mL;绿僵菌素产量最高的分生孢子突变株是08菌株,其DA达到(158.29±3.85)μg/mL,比出发菌株增产48.21%;产量最高的原生质体突变株是24菌株,其DA和DB产量分别达到(146.95±12.28)和(36.91±5.37)μg/mL,比出发菌株分别增产37.59%和25.11%.分生孢子诱变的DA和DB正突变率分别为15.00%和0,负突变率分别达到85.00%和97.50%;原生质体诱变的DA和DB正突变率分别为17.50%和5.00%,负突变率分别为70.50%和95.50%.原生质体诱变效果优于分生孢子诱变效果.另外,紫外线诱变不会改变DA与DB产量的正相关性.

By using the Metarhizium anisopliae var. ansopliae strain MaQ10 as an original strain and the technics of ultraviolet ray （UV） inducing the conidia spores and protoplasts, fourty mutants of spores or protoplasts were selected and subjected to investigating for the changes of the destruxins production. The original strain MaQ10 yielded 106.81 and 29.53 μg/mL for destruxins A and B （DA and DB）, fourty spores mutants yielded DA and DB for （45.05±7.77） and （ 10. 83 ± 1. 05） μg/mL, while the mutants of protoplasts got an average production of DA and DB for （ 68. 17 ± 6.07 ） 和 （ 11. 80± 1. 35）μg/mL, respectively. The best producer of destruxins from spores mutants was the strain 08 which produced DA （ 158.29 -＋3.85） μg/mL, and increased by 48.21% compared to MaQI0 strain. The best producer of destruxins from protoplast mutants was 24 strain, its yields of DA and DB were （ 146. 95± 12. 28 ） and （36.91 ±5.37） μg/mL, which increased by 37.59% and 25.11% , respectively. Furthermore, conidia spores had the 15.00% and 0 positive mutation rate for DA and DB, the 85.00% and 97.50% of negative mutation rate for DA and DB ; meanwhile protoplasts got a higher positive rate 17.50% and 5.00% for DA and DB, which had a lower negative rate 70. 50% and 95.50% , respectively. It was suggested that inducing protoplasts was more effective than inducing conidia spores, and the treatment with UV could not change the positive correlationship of production between DA and DB.