绿僵菌相容性杀虫剂筛选及混用防治椰心叶甲

Screening of Chemical Pesticides Compatible with Metarhizium anisopliae for Control of Brontispa longissima

根据6种常用杀虫剂的常规使用浓度、亚致死浓度、次亚致死浓度对金龟子绿僵菌Metarhizium anisopliae M09菌株菌落生长的影响结果，筛选了与绿僵菌相容的杀虫剂，并选用适当剂量的杀虫剂与绿僵菌混用，防治椰心叶甲Brontispa longissima．结果表明，供试的6种杀虫剂对绿僵菌生长有不同程度的抑制作用，其中杀虫单的抑制作用最弱，亚致死剂量药剂5d后对菌落生长的抑制率为15％，10d后下降到5．90％，次亚致死剂量5d和10d对菌落生长的抑制率均低于5％，与绿僵菌有较好的相容性．低剂量杀虫单与绿僵菌混用防治椰心叶甲成虫具有协同作用，亚致死剂量和次亚致死剂量的杀虫单和绿僵菌混用，防治椰心叶甲的致死率分别达93．33％和81．11％，要明显高于单独使用绿僵菌（75．56％）和单独使用亚致死和次亚致死剂量杀虫单（10％～30％），具有较好的应用前景．

To screen the compatible chemical pesticide with entopathogenic fungus Metarhizium anisopliae strain M09, which is high virulent to Brontispa longissima, six chemical pesticides were tested. The commen concentration, sublathal concentration and low sublathal concentration were used to compare the compatibility of the chemical pesticides. The results indicated that the molosultap was the best pesticide compatible with M09 for its less inhibition to the colony growth. 15% of inhibition for M09 colony growth rate was observed when growing on PPDA culture medium mixed with sublethal dose of molosultap in 5 d and 5.9% of inhibition in 10 d after inoculation, while that of low sublethal dose was less than 5%. The results of interactive effect of M. anisopliae and sublethal dose of molosultap against B. longissima indicated that the mixture had synergetic effect in controlling B. longissima. The lethal mortality of M09 mixed with sublethal dose and low sublethal dose molosultap were 93.33% and 81.11% respectively, higher than M09 or molosultap when they were used independently.