表皮生长因子对牛眼小梁细胞c-fos基因表达的诱导

The expression of c fos gene in cultured bovine trabecular cells induced by epidermal growth factor

目的研究表皮生长因子（ｅｐｉｄｅｒｍａｌｇｒｏｗｔｈｆａｃｔｏｒ，ＥＧＦ）对小梁细胞的作用。方法应用分子杂交技术研究ＥＧＦ对体外培养的牛眼小梁细胞ｃ－ｆｏｓ基因表达的诱导和３Ｈ胸腺核苷酸（３Ｈ－ｔｈｙｍｉｄｉｎｅｉｎｃｏｒｐａｒａｔｉｏｎ，３Ｈ－ＴｄＲ）掺入法观察细胞ＤＮＡ的合成。结果小梁细胞的３Ｈ－ＴｄＲ掺入率随着ＥＧＦ浓度不同而变化，浓度为２０～１５０ｎｇ／ｍｌ时，细胞掺入率随浓度增加升高（Ｐ＜０．０１）。与对照组相比，ＥＧＦ刺激停止生长的小梁细胞０．５小时后，ｃ－ｆｏｓ基因开始表达，１小时后达高峰，至２小时后消失。不同浓度ＥＧＦ刺激小梁细胞１小时后，ｃ－ｆｏｓ基因表达呈浓度依赖性。结论实验结果表明ＥＧＦ刺激小梁细胞增殖或进入生长状态，可能与ｃ－ｆｏｓ基因产物的信号传递作用有关。

Objective To investigate the effect of epidermal growth factor (EGF) on cultured trabecular cells. Methods Molecular hybridization technique was used to investigate the effect of EGF on c fos expression and its induction of bovine trabecular cells in vitro, and 3H TdR incorporation method was applied to study the synthesis of DNA. Results EGF enhanced the incorporation of 3H TdR into trabecular cells at concentrations ranging from 20～150 ng/ml ( P <0.001). Compared with control, the expression of c fos appeared at 0.5 hour and 1 hour after stimulation, and did not exist till 2 hr. The level of c fos expression in cells relied on the concentration of EGF. Conclusion Our results suggest that EGF stimulate trabecular cell proliferation at least in part by the regulation of the c fos gene and its nuclear protein.