轻度低温对大鼠缺血性脑损伤保护作用观察

PROTECTIVE EFFECTS OF MILD POSTISCHEMIC HYPOTHERMIA ON RATS OF GLOBAL CEREBRAL ISCHEMIA

观察了再灌流期３３°Ｃ低温对大鼠脑缺血１０ｍｉｎ的保护作用，发现低温组神经系统损害积分明显低于正常体温组；水迷宫试验低温组游完全程的时间及错误次数显著低于正常体温组。组织病理学显示，正常体温组海马ＣＡ１段神经元损害率明显高于低温组。提示短暂脑缺血后，再灌流期轻度低温对脑组织及行为有明显保护作用。

We investigated the effects of mild postischemic hypothermia on behavior and neurophathologic outcome in rats. Three groups of animals were investigated:(1) rates subjected to 10 min of four vessel occlusion(4VO) under normothermia;(2)rats subjected to 10min of 4VO followed by 33°C hypothermic reperfusion for 3h; (3) control rats not subjected to ischemia. The scoring scale of brain injury and water maze test showed that there were more severe behavioral deficits in normothemic rats. The percentage of necrotic neurons in CA 1 was analysed 7 days after ischemia. Hypothermic rats exhibited significantly lower necrotic neurons(38 9%) in CA 1 compared to the normothermic rats (91 7%).The results indicate that mild postischemic hypothermia provide protection from behavioral deficits and neuronal injury following transient global cerebral ischemia.