蔺草基因组DNA提取方法的比较研究

COMPARATIVE STUDY OF EXTRACTION METHOD FOR GENOMIC DNA IN MAT RUSH

以16个蔺草品种实生苗和4个蔺草品种组培苗为材料,探讨了CTAB改良法与DNA试剂盒法对蔺草基因组DNA提取质量的影响。结果发现,对于同一品种而言,CTAB改良法提取获得的DNA A260nm和OD值远低于试剂盒提取法,前者的DNA浓度仅为0.65-4.90μg/ml,而后者则为5.10-32.80μg/ml,后者为前者的1.6-21.2倍。这表明试剂盒提取法更适合于蔺草基因组DNA的提取。试验还发现提取材料对基因组DNA的质量有显著影响,蔺草组培苗优于实生苗。

Seedlings of sixteen mat rush varieties planted in the field, and shoots derived from tissue culture of four varieties were used to investigate the effects of modified CTAB method and Isolation Kit method on the extraction quality of genomic DNA. The results showed that A260nm, and OD values of DNA solution of same material extracted by modified CTAB method were lower than those by isolation kit method. The concentrations of DNA solution were 0.65 - 4.90 μg/ml with the former method and 5,10- 32.80 μg/ml with the latter method. Therefore, it was demonstrated that DNA Isolation Kit method was more suitable for genomic DNA extraction of mat rush. In addition, it was also found that extraction material had the significant impact on the extraction quality of genomic DNA, and the shoots developed by tissue culture were better than seedlings planted in the field.